Figure 3

Temperature-dependent in vitro c-Fos expression in the cord. Tissue sections were counterstained with cresyl violet. Photomicrographs show the dorsal quadrant of transverse sections of T3 from the cord with 3 h incubation in ≤ 10°C (A) or 25°C (B) normal aCSF. In the cord incubated at ≤ 10°C, only a few c-Fos-positive cells were found in the IML (Aii) and around the cc (Aiv). In the cord incubated at 25°C, abundant c-Fos-positive cells concentrated in the IML (Bii) and the lamina X (Biii); some were scattered throughout the dorsal laminae (Bi) or the lfu (Bii), and accumulated in the outer rim of the white matter (B, Bii). A few c-Fos positive cells scattered around the cc were observed in the cord incubated at ≤ 10°C (Aiv), which were absent when the cord was incubated at 25°C (Biv). Scale bars = 150 μm in (A, B) and 60 μm in (Ai-iv, Bi-iv).