Figure 2

Fraction of unfolded apoNCS at various concentrations of GdnHCl. The GdnHCl-induced unfolding at 25°C was monitored by changes in fluorescence emission at 340 nm (excited at 280 nm) (open circle) and ellipiticity at 224 nm (black circle). The concentration of apoNCS used in the fluorescence and far-UV CD experiments was 5 and 15 μM, respectively. Samples were prepared in 10 mM phosphate buffer at pH 3.