Figure 5
![Figure 5](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2F1423-0127-16-48/MediaObjects/12929_2009_Article_48_Fig5_HTML.jpg)
Thermal unfolding of apoNCS at the intermediate state. Heat-induced unfolding of apoNCS was monitored by changes in ellipticity at 224 nm in the absence (black circle) and presence (open circle) of 1.2 M GdnHCl. ApoNCS samples (25 μM) were prepared in 10 mM phosphate buffer at pH 3.