Effects of anti-oxidants on ACh-induced increases in [Ca2+]
with LPC. Fura-2 loaded endothelial cells were treated with the anti-oxidants ascorbic acid, PDTC and BHT for 30 min. ACh (3 μmol/L) was added before and after treatment. Data are expressed as percentage value of ACh-induced increases in calcium prior to the treatment. Each value represents the mean ± S.E. of 7 cells. ***, P < 0.001 compared between indicated groups.