Figure 7

Proposed catalytic mechanism of EndoG. His-141 acts as a general base to activate a water molecule, generating a nucleophile that attacks the phosphodiester bond. Arg-110 and magnesium ion stabilize the transition state.
Proposed catalytic mechanism of EndoG. His-141 acts as a general base to activate a water molecule, generating a nucleophile that attacks the phosphodiester bond. Arg-110 and magnesium ion stabilize the transition state.