Skip to main content

Table 2 Steady-state kinetic data for human EndoG variants.

From: Mutagenesis identifies the critical amino acid residues of human endonuclease G involved in catalysis, magnesium coordination, and substrate specificity

EndoG variants K m (nM)a k cat (S-1)a k cat/K m (S·nM-1)a
Wild type 18.72 ± 1.84 0.07 ± 0.01 3.58 × 10-3 ± 3.11 × 10-4
R110A n.d. n.d. n.d.
R139A 1.01 ± 0.03 2.77 × 10-5 ± 4.44 × 10-7 2.75 × 10-5 ± 1.47 × 10-6
H141A 12.93 ± 2.17 2.48 × 10-6 ± 4.34 × 10-7 1.96 × 10-7 ± 4.78 × 10-8
H141D 1.94 ± 0.05 9.68 × 10-6 ± 2.59 × 10-13 5.03 × 10-6 ± 1.72 × 10-7
N163A 8.51 ± 2.28 7.52 × 10-6 ± 1.41 × 10-6 9.05 × 10-7 ± 7.67 × 10-8
N163K 12.10 ± 2.03 2.35 × 10-6 ± 1.85 × 10-7 1.99 × 10-7 ± 1.61 × 10-8
N172A 8.75 ± 0.13 3.77 × 10-5 ± 4.26 × 10-8 4.31 × 10-6 ± 5.95 × 10-8
R184A 1.53 ± 0.02 7.68 × 10-3 ± 3.27 × 10-10 5.03 × 10-4 ± 1.91 × 10-5
H228A 6.01 ± 1.88 5.34 × 10-3 ± 2.71 × 10-3 8.61 × 10-4 ± 1.81 × 10-4
H228D 5.70 ± 0.33 4.74 × 10-4 ± 1.78 × 10-5 8.33 × 10-5 ± 1.75 × 10-6
N251A 3.13 ± 0.37 3.15 × 10-6 ± 5.68 × 10-14 1.02 × 10-6 ± 6.64 × 10-8
E271A n.d. n.d. n.d.
R272A 2.30 ± 0.20 3.67 × 10-3 ± 9.23 × 10-5 1.61 × 10-3 ± 9.96 × 10-5
H141A/H228A 8.06 ± 1.92 1.18 × 10-7 ± 1.26 × 10-8 1.48 × 10-8 ± 1.98 × 10-9
  1. a Data are presented as mean ± standard error of triplicate assays.
  2. n.d., not determined.