|
Wild type
|
18.72 ± 1.84
|
0.07 ± 0.01
|
3.58 × 10-3 ± 3.11 × 10-4
|
|
R110A
|
n.d.
|
n.d.
|
n.d.
|
|
R139A
|
1.01 ± 0.03
|
2.77 × 10-5 ± 4.44 × 10-7
|
2.75 × 10-5 ± 1.47 × 10-6
|
|
H141A
|
12.93 ± 2.17
|
2.48 × 10-6 ± 4.34 × 10-7
|
1.96 × 10-7 ± 4.78 × 10-8
|
|
H141D
|
1.94 ± 0.05
|
9.68 × 10-6 ± 2.59 × 10-13
|
5.03 × 10-6 ± 1.72 × 10-7
|
|
N163A
|
8.51 ± 2.28
|
7.52 × 10-6 ± 1.41 × 10-6
|
9.05 × 10-7 ± 7.67 × 10-8
|
|
N163K
|
12.10 ± 2.03
|
2.35 × 10-6 ± 1.85 × 10-7
|
1.99 × 10-7 ± 1.61 × 10-8
|
|
N172A
|
8.75 ± 0.13
|
3.77 × 10-5 ± 4.26 × 10-8
|
4.31 × 10-6 ± 5.95 × 10-8
|
|
R184A
|
1.53 ± 0.02
|
7.68 × 10-3 ± 3.27 × 10-10
|
5.03 × 10-4 ± 1.91 × 10-5
|
|
H228A
|
6.01 ± 1.88
|
5.34 × 10-3 ± 2.71 × 10-3
|
8.61 × 10-4 ± 1.81 × 10-4
|
|
H228D
|
5.70 ± 0.33
|
4.74 × 10-4 ± 1.78 × 10-5
|
8.33 × 10-5 ± 1.75 × 10-6
|
|
N251A
|
3.13 ± 0.37
|
3.15 × 10-6 ± 5.68 × 10-14
|
1.02 × 10-6 ± 6.64 × 10-8
|
|
E271A
|
n.d.
|
n.d.
|
n.d.
|
|
R272A
|
2.30 ± 0.20
|
3.67 × 10-3 ± 9.23 × 10-5
|
1.61 × 10-3 ± 9.96 × 10-5
|
|
H141A/H228A
|
8.06 ± 1.92
|
1.18 × 10-7 ± 1.26 × 10-8
|
1.48 × 10-8 ± 1.98 × 10-9
|
