Table 2 Steady-state kinetic data for human EndoG variants.
EndoG variants | K m (nM)a | k cat (S-1)a | k cat/K m (S·nM-1)a |
|---|---|---|---|
Wild type | 18.72 ± 1.84 | 0.07 ± 0.01 | 3.58 × 10-3 ± 3.11 × 10-4 |
R110A | n.d. | n.d. | n.d. |
R139A | 1.01 ± 0.03 | 2.77 × 10-5 ± 4.44 × 10-7 | 2.75 × 10-5 ± 1.47 × 10-6 |
H141A | 12.93 ± 2.17 | 2.48 × 10-6 ± 4.34 × 10-7 | 1.96 × 10-7 ± 4.78 × 10-8 |
H141D | 1.94 ± 0.05 | 9.68 × 10-6 ± 2.59 × 10-13 | 5.03 × 10-6 ± 1.72 × 10-7 |
N163A | 8.51 ± 2.28 | 7.52 × 10-6 ± 1.41 × 10-6 | 9.05 × 10-7 ± 7.67 × 10-8 |
N163K | 12.10 ± 2.03 | 2.35 × 10-6 ± 1.85 × 10-7 | 1.99 × 10-7 ± 1.61 × 10-8 |
N172A | 8.75 ± 0.13 | 3.77 × 10-5 ± 4.26 × 10-8 | 4.31 × 10-6 ± 5.95 × 10-8 |
R184A | 1.53 ± 0.02 | 7.68 × 10-3 ± 3.27 × 10-10 | 5.03 × 10-4 ± 1.91 × 10-5 |
H228A | 6.01 ± 1.88 | 5.34 × 10-3 ± 2.71 × 10-3 | 8.61 × 10-4 ± 1.81 × 10-4 |
H228D | 5.70 ± 0.33 | 4.74 × 10-4 ± 1.78 × 10-5 | 8.33 × 10-5 ± 1.75 × 10-6 |
N251A | 3.13 ± 0.37 | 3.15 × 10-6 ± 5.68 × 10-14 | 1.02 × 10-6 ± 6.64 × 10-8 |
E271A | n.d. | n.d. | n.d. |
R272A | 2.30 ± 0.20 | 3.67 × 10-3 ± 9.23 × 10-5 | 1.61 × 10-3 ± 9.96 × 10-5 |
H141A/H228A | 8.06 ± 1.92 | 1.18 × 10-7 ± 1.26 × 10-8 | 1.48 × 10-8 ± 1.98 × 10-9 |