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Figure 1 | Journal of Biomedical Science

Figure 1

From: Lipopolysaccharide preconditioning enhances the efficacy of mesenchymal stem cells transplantation in a rat model of acute myocardial infarction

Figure 1

VEGF concentration in the culture supernant of MSCs. ELISA analysis of supernatant after stimulation of LPS is shown. Wild MSCs were exposed to increasing doses of LPS for 48 hours. VEGF concentration increased after LPS treatment and VEGF concentration was the highest after incubation with 1.0 μg/ml LPS. B. In the presence of NF-κB inhibitor-PDTC, LPS stimulation caused a subsequent decrease in VEGF expression compared with that without NF-κB inhibition. LPS stimulation on tMSCs caused an obvious decrease in VEGF expression compared with that of wild MSCs. (a. VEGF concentration of wMSCs without stimulation of LPS; b. VEGF concentration of wMSCs with 1.0 μg/ml LPS stimulation; c. VEGF concentration of wMSCs with stimulation of 1.0 μg/ml LPS and 5 nmol/L PDTC; d. VEGF concentration of TLR4 gene deleted MSCs with stimulation of 1.0 μg/ml LPS) Data are mean ± SEM. *P < 0.05 vs. control group, #P < 0.05 vs. NF-κB inhibition and TLR4 deletion groups.

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Journal of Biomedical Science

ISSN: 1423-0127