Figure 5

Determination of apoptosis. The nerve tissues implanted with AFS and treated with or without Natto were obtained 7 days after injury and subjected to apoptotic assay by TUNEL in the AFS (A-B) and crushed nerve (C-D) groups. (E, F) Hoechst 33342 staining in AFS corresponded to the areas in (A) and (B). (G, H) DAPI staining in the injured nerve corresponded to areas in (C) and (D). Magnification box indicated the merged imaging of TUNEL with S-100 (red). The quantitative analysis of TUNEL in the AFS and injured nerve groups is shown in (I). * p < 0.05, *** p < 0.001, bar length = 50 μm, n = 6.