Figure 2

(a) Effects of armepavine on TNF-α-induced IκBα phosphorylation in the cytoplasmic extract and translocation of NFκB in the nuclear extract of HSC-T6 cells for 6 hrs, with PCNA and α-tubulin as internal controls. (b) Effects of armepavine on TNF-α-induced NFκB transcriptional activity using luciferase reporter gene assay in HSC-T6 cells for 6 hrs, with N -acetylcysteine (NAC) as a positive control. Representative results from three independent experiments are shown here *p < 0.05 for TNF-α alone vs. Control; ^#p < 0.05 for TNF-α + armepavine or NAC vs. TNF-α alone.