Figure 2
From: Increased ATP generation in the host cell is required for efficient vaccinia virus production
Western blotting analysis of drug effects on ND4 expression in VV infected cells. (A) HeLa cells were infected with VV (MOI = 1) for the indicated times in the presence or absence of 45 μM apigenin. Actin was used as a loading control. (B) The ND4 up-regulation by vaccinia virus infection (MOI = 1) was inhibited by the DNA polymerase inhibitor, ara C. Cells were incubated with ara C (40 μg/ml), an inhibitor of poxviral DNA synthesis, 30 min before VV infection and throughout the infection. The ND4 protein is indicated with an arrow while non-specific bands are indicated by thin lines. ERK2 protein was used as a loading control.