Figure 5
From: Increased ATP generation in the host cell is required for efficient vaccinia virus production
(A) The intracellular ATP concentration of vaccinia virus infected (MOI = 1) and oligomycin-treated cells. ATP concentration was measured 13 hrs after vaccinia virus infection in the presence of various concentration of oligomycin. Experiments were performed three times in duplicate. (B) Western blotting analysis of Vaccinia viral protein expression (A type inclusion protein and IMV heparin binding surface protein) in the presence of 4.5 μM oligomycin. ERK2 protein was used as a loading control. (C) Both the intracellular and the extracellular vaccinia viruses were reduced 13 hr after virus infection in the presence of 4.5 μM oligomycin. Experiments of plague assay were performed three times in triplicate.