Skip to main content
Figure 8 | Journal of Biomedical Science

Figure 8

From: Myofibril-Inducing RNA (MIR) is essential for tropomyosin expression and myofibrillogenesis in axolotl hearts

Figure 8

Determination of myofibril protein expression. Real-time RT-PCR confirms the knockdown of endogenous MIR (A) in the normal hearts but shows no significant difference at the level of the transcripts of alpha- (B) and TM4 type tropomyosin (C) transcripts in the normal hearts treated with double-stranded MIR (dsRNA-) for 36 hours or 84 hours. However, both alpha- and TM4 type tropomyosin transcripts in the normal hearts treated with dsRNA for 9 days show dramatic decreases to nearly 1/3 of the levels in control groups with hearts incubated in lipofectamine transfection medium only. MIR expression in 9-day-cultured hearts is not detectable.

Back to article page

Journal of Biomedical Science

ISSN: 1423-0127