Antibacterial activity assay of rHMGB1, tHMGB1, rHMGB1 A box, B box, and C peptide with the method of dilution in the test tubes. The antibacterial activities of rHMGB1, tHMGB1, rHMGB1 A box, B box, and C peptide were compared by the method of dilution in the test tubes as described in the Materials and Methods. The bacteria used in this experiment included SA, JM109, ATCC 25922, DH5α and PA. Each experiment was performed in triplicate and repeated three times. DHFR protein purified by the same system was used as a negative control. Data showed as mean ± standard deviation and were analyzed with One-Way ANOVA. * P < 0.05 vs control DHFR.