Figure 8

MAPK signaling pathways were involved in J96-induced AP-1 activation of uroepithelial cells. Type 1 fimbriated J96 (J96-1) induced AP-1 (A) and NF-κB (C) activation in 5637 cells, and P fimbriated J96 (J96-P) induced AP-1 (B) and NF-κB (D) activation in 786-O cells were determined by TF ELISA assays in cells pretreated with vesicle (DMSO), PD98059 (PD; 30 μM), SP600125 (SP; 20 μM), or SB203580 (SB; 10 μM) individually for 1 h, or transfected with si-CL, si-p38, and then invaded with J96 for 2 h. The results are shown as mean ± SEM. * P < 0.05 versus CL. ^#P < 0.05 versus DMSO-treated cells with J96 invasion. ^&P < 0.01 versus DMSO-treated or si-CL transfected cells with J96 invasion.