Figure 3

Knockdown of ODC-AS by RNA interference. A. Phenotype changes in adipogenesis by shRNA knockdown of ODC-AS. Microphotographs of 3T3-L1 cells infected with shRNA (ODCi-1, ODCi-3 and ODCi-5) for 3 days before differentiation cocktail was added, and the cells were stained with Nile Red 6 days after differentiation. Nsi. Non-specific shRNA served as a negative control; PPARγi is a shRNA designed specifically against PPARγ, served as a positive control to inhibit 3T3-L1 differentiation. B. Quantification of intracellular triglyceride in ODCi-5-infected 3T3-L1 and Nsi control cells. Cells were infected 3 days before differentiation and samples were collected 6 days after differentiation. Data shown are means ± S.D. and were in triplicates of three separate experiments. C. Quantification of intracellular triglyceride in ODCi-5-infected, Nsi-infected 3T3-L1cells and normal control (NC, non-infected). The cells were infected 2 days after the induction of differentiation and samples were collected 10 days after differentiation. Data shown are means ± S.D. and were in triplicates of three separate experiments. * indicates P < 0.01, Student t test.