Figure 3
Oligomycin-induced Cellular ROS Production and Cell Death in HT-22 Cells. A: Flow cytometric analysis of cellular ROS levels with the simultaneous use of CM-H2DCFDA and MitoSOX red and the effect of oligomycin treatment (10 μg/ml, 2 hr). DCF: dichlorofluorescein. MFI: median fluorescence intensity. B: Phase contrast images. Images were taken after the cells were treated or untreated with 10 μg/ml oligomycin for 8 hr. C: JC-1 staining. The cells were treated or untreated with 10 μg/ml oligomycin for 2 hr.