Figure 2

Fractionation profile of distinct FACT-MCM complexes. HeLa nuclear extracts (Figure 1A) were subjected to gel filtration chromatography using Sephacryl S-400. Generated fractions (numbers as indicated) were immuno-probed with the specified antibodies. Selected fractions were further subjected to immunoprecipitation using 10D1 and 8D2, and subsequently probed with the indicated antibodies. Peaks 1, 2, and 3, as shown in the 10D1 IP and 8D2 IP panels, denote the approximate fractions in which copurification of MCM with FACT was observed.