Figure 6

The FACT-MCM interaction is reduced in response to growth inhibition. Immunoblot showing the levels of FACT, MCM4, and MCMs in K562 cell extracts (lanes 1 & 2) and in 10D1 immunoprecipitates (lanes 3 & 4). Before extraction, K562 cells were either uninduced (lanes 1 & 3) or induced to differentiate with 3-day treatment of 2 mM sodium butyrate (NaBu) (lanes 2 & 4). The number at the bottom illustrates the relative levels of MCM co-immunoprecipitated by the 10D1 antibody. The degree of co-IP ("relative MCM co-IP"), expressed as a ratio in band intensity of total MCM to FACT, was normalized to the control treatment group.