Figure 1

Expression of LMO2-L and -S isoform and activities of their promoters in haematopoietic and non-haematopoietic cells. (A) Sketch map of three transcripts of LMO2. Lmo2-a/b which have 6 and 4 exons respectively encode LMO2-L, and Lmo2-c that has 2 exons encodes LMO2-S. The 3 black bars represent 3 promoters P1, P2 and P3, the regions used for reporter assays were marked below. (B) Difference in protein sequences between LMO2-L/S. The cartoon shows the difference in N-terminal regions and the different amino-acids are shown beneath. (C) Real-time PCR detection of LMO2-L/S expression in cell lines. The bars represented the ratio between relative expression levels of LMO2-L/S and GAPDH, the data showed the means of three separate experiments. (D) Relative activity of P1, P2 and P3. pGL4-basic was used as control and its activity was marked as 1. All the data came from at least three independent experiments and were evaluated by student's t-test, p < 0.05.