Diagram showing the experimental design. On Day 1, seven WT and seven ppENK mice received footshocks (3 mA) for 10 s to induce a traumatic event. Nine WT and nine ppENK mice received no footshocks. On Days 2, 7, and 13, all mice were exposed to situational reminders, and freezing behavior was recorded. On Day 14, each mouse underwent the following behavioral tests: open field test, elevated plus maze, light/dark avoidance test, and forced swim test. Two hours later, mice were euthanized and examined for Fos immunolabeling in multiple brain areas.