Figure 4

ADAM10 is expressed in podocytes in human renal tissue. (A) Glomeruli from human kidney were isolated and lysed and investigated by an ADAM10 specific westernblot (left panel). Right panel, double immunofluorescence analysis on a human kidney section with WT1 (red) and ADAM10 (green) antibodies, demonstrating ADAM10 expression in WT1 positive podocytes. (B) Increased ADAM10 levels are found in the urine of patients with glomerular kidney diseases. Western Blot analysis of ADAM10 expression in urine and urinary vesicles of healthy volunteers (HV 1-2) and patients with glomerular kidney diseases (number of patients P1-5, ADAM10 expression in supernatants (SN) and vesicles (VES) from untreated (HPC C) or treated with 1 μM ionomycin (HPC IONO) for 24 h. Membranes were reprobed with CD9 an specific marker protein of exosomes.