Functional classification and subcellular localization of identified proteins based on bioinformatics. Regulated proteins identified by 2-DE were analyzed further by Gene Ontology (GO) analysis and Ingenuity Pathway Analysis (IPA). GO analysis for functional classification of proteins identified by proteomics. Catalysis and protein binding are major functions of the identified proteins (A). IPA of subcellular localization of identified proteins, most of which are located in cytoplasm (B).