Figure 3
From: Trypsin-induced proteome alteration during cell subculture in mammalian cells
Representative immunoblotting analysis for selected differentially expressed proteins during trypsinization. The levels of identified proteins (A) tropomyosin alpha-1, (B) HSP-60, (C) SCaMC-1, (D) VDAC2, (E) VDAC1, and (F) CD63 in MCF-7 cells were analyzed by immunoblotting (left top panels), protein expression map from 2D-DIGE (left middle panels), three-dimensional spot images (left bottom panels) and relative quantification of western blotting and 2D-DIGE data for each target protein (right panels).