Figure 1
From: Sonoporation-mediated gene transfer into adult rat dorsal root ganglion cells
Optimal conditions for DRG transfection via sonoporation. Dissociated cells from adult rat DRGs were suspended at a density of 10,000 cells per cm3 in a solution containing 10 μg/ml of pE-GFP C1 and were sonicated for 1 s at 2.5-10 W using a 12 mm diameter probe tip. The cells were maintained in vitro for 48 h after sonoporation. The calibration bar in Panel c represents 100 μm and applies to Panels a-c. Panel d shows the number of GFP-expressing cells per 5 mm2 after sonication at various energy levels. Panel e shows the number of GFP-expressing cells per 5 mm2 after sonication for various sonication durations at 5 W.