Effects of simvastatin on (A) thromboxane B
formation, (B) phospholipase Cγ2 and (C and D) PKC substrate (p47) phosphorylation in activated platelets. Washed platelets were preincubated with simvastatin (30 and 50 μM) or 0.5% DMSO, followed by the addition of collagen (1 μg/ml) or PDBu (150 nM) to trigger platelet activation. Cells were collected, and subcellular extracts were analyzed for (A) thromboxane A2 formation, (B) phospholipase Cγ2 phosphorylation, and (C and D) phospho-PKC substrate (p-p47) as described in "Methods". Data are presented as the means ± S.E.M. (n = 4); *P < 0.05 and **P < 0.01, compared to the control group; #P < 0.05 and ##P < 0.01, compared to the collagen group.