Figure 4

HSV-1 infection alters the levels of p63 and Bax-β in the SIRC cell line. Total protein was isolated from mock-infected cells and from cultures infected with HSV-1 at MOIs of 0.001, 0.01, 0.1, 1 and 10 at the indicated time points. Samples were resolved on SDS-PAGE and transferred onto nitrocellulose filters. The steady-state levels of gD, Bax-α, Bax-β and p63 were analyzed by Western blot assay. To determine protein levels in HSV-1-infected cells, band intensities were quantified by use of the ImageQuant software. The numbers indicate the relative quantities of each band, normalized to the control cells at each time point. Lanes 1, 6, 11, 16, 21 and 26, mock-infected cells; lanes 2-5, 7-10, 12-15, 17-20, 22-25 and 27-30, HSV-1-infected cultures. The results are representative of three independent experiments.