The HSV-1-mediated TAp63γ expression requires viral DNA replication. SIRC cells were infected with the KOS strain of HSV-1 at an MOI of 10 and maintained for 24 h in the absence or in the presence of Acyclovir (ACG). To determine the dependence of the TAp63γ expression on HSV-1 DNA replication, the levels of gD, Bax and p63 were determined by Western blot assay. To determine protein levels in HSV-1-infected cells, band intensities were quantified by densitometric analysis with the Imagequant software. The numbers indicate the relative quantities of each band, normalized to the control cells at each time point. Lane 1: HSV-1-infected cells incubated in the absence of ACG; lanes 2-4: HSV-1-infected cells incubated in the presence of ACG. The results are representative of three independent experiments.