Effect of deleting l0045 on the levels of representative TTS proteins. (A) Comparison of representative TTS proteins detected in the bacterial lysates and the culture media. (B) Similar comparison of the representative proteins as in (A) except that bacteria were transformed with the specified plasmids. Bacteria were cultivated in M9 for 6 h in the presence of 5% CO2, harvested by centrifugation and disrupted by suspending in SDS sample buffer whereas the spent media were filtered and then concentrated by TCA precipitation. Protein samples were run in SDS-PAGE and examined by Western blotting. Note: pACYC184_L45 was derived from pACYC184 by inserting fragment containing l0045 with its own upstream promoter. OmpC from the bacterial outer membrane was also detected to assure a comparable sample loading.