Tight regulation of l0045 in EHEC. (A) Comparison of growth curves of plasmid-transformed bacteria. Upper panel: K-12 (JM109); lower panel: wild-type EHEC. IPTG was added to the media as marked during the cultivation. (B) L0045 detected in the total lysates of bacteria harvested from (A) after 3-h IPTG induction. Loadings of bacterial lysates were comparable as seen with the internal control of OmpC.