Analyzing molecular determinant that causes L0045 differently seen between EHEC and JM109. (A) & (B), total proteins from bacteria examined for the plasmid-encoded L0045 by SDS-PAGE in conjunction with Western blotting using anti-Hisx6. OmpC was detected in parallel by anti-OmpC for the purpose of comparable loading control. (C) Comparative RT-PCR to detect l0045-mRNA in EHEC strains that carry the specified plasmids. OmpC, as internal control to normalize the amount of mRNA.