Figure 2
Effect of hnRNP A1 on the synthesis of G and SG RNA in infected cells. (A) Western blot to examine the expression of hnRNP A1. hnRNP A1 was depleted by siRNA knockdown technique as described under Materials and Methods. Cell lysates were prepared 3 days after transfection of siRNA. Western blot was carried out using antibody to hnRNP A1. Expression of actin was examined by Western blot as a control. (B) Northern blot to detect G and SG RNA in infected cells. Cells were transfected with no siRNA or siRNA targeting hnRNP A1 for 3 days. Cells were infected with SV at an moi of 40 pfu/cell for 8 h. Total RNA were extracted and electrophoresed through an agarose gel, transferred to a nylon membrane, and characterized by Northern blot analysis, using 32P-labeled negative-strand probe SV7772(-). (C) Replication of SV in hnRNP A1-depleted cells. Cells treated or untreated with siRNA targeting hnRNP A1 were infected with SV at an moi. of 40 pfu/cell and incubated at 34°C. Medium was harvested 8 h p.i. and assayed for infectious virus by plaque formation on CEF cells. Mean values and standard errors from duplicate samples are shown.