Table 1 Bacterial strains and plasmids used in this study

Strains

   K. pneumponiae

CG43S3

CG43 Sm^r

[14]

ΔpmrF

CG43S3ΔpmrF Sm^r

This study

ΔphoP

CG43S3ΔphoP Sm^r

This study

ΔpmrD

CG43S3ΔpmrD Sm^r

This study

ΔpmrA

CG43S3ΔpmrA Sm^r

This study

Δugd

CG43S3Δugd Sm^r

This study

Δwza

CG43S3Δwza Sm^r

This study

ΔlacZ

CG43S3ΔlacZ Sm^r

[35]

ΔlacZ ΔphoP

CG43S3ΔlacZ ΔphoP Sm^r

This study

ΔlacZ ΔpmrD

CG43S3ΔlacZ ΔpmrD Sm^r

This study

ΔlacZ ΔpmrA

CG43S3ΔlacZ ΔpmrA Sm^r

This study

ΔpmrA ΔphoP

CG43S3ΔpmrA ΔphoP Sm^r

This study

ΔrcsB (B2202)

CG43S3ΔrcsB Sm^r

[14]

ΔpmrA ΔrcsB

CG43S3ΔpmrA ΔrcsB Sm^r

This study

ΔpmrD ΔrcsB

CG43S3ΔpmrD ΔrcsB Sm^r

This study

ΔphoP ΔrcsB

CG43S3ΔphoP ΔrcsB Sm^r

This study

   E. coli

S17-1λpir

hsdR recA pro RP4-2 (Tc::Mu; Km::Tn7)(λpir)

[34]

XL1-Blue MRF' Kan

Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac [F' proAB lacI^qZ ΔM15 Tn5 (Kan^r)]

Stratagene

BL21(DE3)

F^- ompT hsdS _B (r _B ^-m _B ^-) gal dcm trxB 15::kan (DE3)

Novagen

Plasmids

yT&A

T/A-type PCR cloning vector, Ap^r

Yeastern

pET30b

His-tagged protein expression vector, Km^r

Novagen

pBT

Bait plasmid, p15A origin of replication, lac-UV5 promoter, λ-cI open reading frame, Cm^r

Stratagene

pTRG

Target plasmid, ColE1 origin of replication, lac-UV5 promoter, RNAP αopen reading frame, Tc^r,

Stratagene

pBT-LGF2

Control plasmid containing a fragment encoding the yeast transcriptional activator Gal4 fused with λ-cI, Cm^r

Stratagene

pTRG-GAL11^P

Control plasmid containing a fragment encoding a mutant form of Gal11 protein, called Gal11P, fused with RNAPα, Tc^r

Stratagene

pKAS46

Suicide vector, rpsL, Ap^r, Km^r

[34]

pRK415

Shuttle vector, mob⁺, Tc^r

[36]

placZ15

promoter selection vector, lacZ⁺, Cm^r

[35]

pRK415-PmrF

1.3-kb fragment containing a pmrF allele cloned into pRK415, Tc^r

This study

pRK415-RcsB

1.2-kb fragment containing the entire rcsB locus cloned into pRK415, Tc^r

[39]

pRK415-PmrA

1.1-kb fragment containing a pmrA allele cloned into pRK415, Tc^r

This study

pRK415-PhoP

900-bp fragment containing a phoP allele cloned into pRK415, Tc^r

This study

pRK415-PmrD

550-bp fragment containing a pmrD allele cloned into pRK415, Tc^r

This study

placZ15-PpmrH

500-bp fragment containing the upstream region of the K. pneumoniae pbgP genes cloned into placZ15, Cm^r

This study

placZ15-PpmrD

350-bp fragment containing the upstream region of the K. pneumoniae pmrD genes cloned into placZ15, Cm^r

This study

pET30b-PhoP

711-bp fragment encoding full-length PhoP cloned into pET30b, Km^r

This study

pET30b-PhoPN

447-bp fragment encoding residues 1-149 of PhoP cloned into pET30b, Km^r

This study

pET30b-PmrBC

828-bp fragment encoding residues 90-365 of PmrB cloned into pET30b, Km^r

This study

pET-PmrA

669-bp fragment encoding full-length PmrA cloned into pET29b, Km^r

This study

pET-PmrD

243-bp fragment encoding full-length PmrD cloned into pET29b, Km^r

This study

pBT-PmrA

669-bp fragment encoding full-length RcsB cloned into pBT, Cm^r

This study

pTRG-PmrD

243-bp fragment encoding full-length RcsA cloned into pTRG, Tc^r

This study