Figure 4
Flow cytometry analysis of TC1-GFP tumor cells incubated with doxorubicin-treated TC1 cells mixed together or separated by a transwell membrane. TC1 cells incubated for 2 hrs in the presence of doxorubicin (100 μg/ml) were added to TC1-GFP cells (7 × 105/well) in various amounts according to ratios indicated. Doxorubicin (10 or 100 μg/ml)-treated TC1 cells (7 × 105) were also added to the upper plate of a transwell system with the TC1-GFP cells (7 × 104) in the bottom plate. A control for the transwell experiment in which the doxorubicin-treated TC1 cells were again mixed with TC1-GFP cells was done. At 24 hrs of culture, the cells from the mixture and from the bottom plate of the transwell system were collected and analyzed using flow cytometery. (A and B) Representative flow cytometery data of (A) TC1-GFP cells mixed with differing amounts of doxorubicin-treated TC1 cells and (B) TC1-GFP cells from the bottom well of the transwell system, the control data to the right. The numbers in the upper right hand corner of each dot plot show the percentage of cells that contain GFP and doxorubicin.