Analysis of EV71 2A protease protein. (A) Amino acid sequence of EV71 2A protein. The predicted 9aa TAD (a.a. 27-35) is indicated with red letters. Potential NES (a.a. 31-42) is underlined. The acidic domain (the last fifteen amino acids) is also underlined. (B) Charge distribution of EV71 2A protease: the C-terminus of this protein is highly acidic.