Mitochondrial genotype in vulvar carcinoma - cuckoo in the nest

Klemba, Aleksandra; Kowalewska, Magdalena; Kukwa, Wojciech; Tonska, Katarzyna; Szybinska, Aleksandra; Mossakowska, Malgorzata; Scinska, Anna; Golik, Paweł; Koper, Kamil; Radziszewski, Jakub; Kukwa, Andrzej; Czarnecka, Anna M; Bartnik, Ewa

doi:10.1186/1423-0127-17-73

Journal of Biomedical Science

Table 3 RFLP haplogroup analysis.

From: Mitochondrial genotype in vulvar carcinoma - cuckoo in the nest

	Haplogroup	Polymorphism	Enzyme	Primer F	Primer R	Primer F - sequence	Primer R - sequence	PCR product	RFLP DNA fragments if this haplogroup	RFLP DNA fragments if not this haplogroup
1	H	C7028T	Alu I	6730F	7398R	CTATGATATCAATTGGCTTCC	GGCATCCATATAGTCACTCC	669	342, 158, 139, 30	342, 188, 139
2	U/K	A12308G	Hinf1	11902F	12328R	GCTAGTCCACGTTCTCCT	TTTGGAGTTGCACCAAGAATT	427	162, 158, 59, 48	221, 158, 48
3	K	G9055A	HaeII	8563F	9231R	ACAATCCTAGGCCTACCCG	GATAGGCATGTGATTGGTGG	669	669	494, 175
4	I	G16398A	BamHI	15879F	16545R	AATGGGCCTGTCCTTGTAG	AACGTGTGGGCTATTTAGGC	667	511, 156	667
5	I	T10031C	Alu I	9821F	10516R	ACTTCACGTCATTATTGGCTC	ATGGAGATGGTAATTGCTAG	696	283, 209, 204	413, 283
6	I	G8251A	AvaII	7960F	8641R	ATTATTCCTAGAACCAGGCG	TGATGAGATATTTGGAGGTGG	682	392, 290	682
7	I	A4529T	HaeII	4184F	4869R	TCCTACCACTCACCCTAGC	GTCATGTGAGAAGAAGCA	686	686	350, 336
8	W	G8994A	HaeIII	8563F	9231R	ACAATCCTAGGCCTACCCG	GATAGGCATGTGATTGGTGG	669	266, 205, 187, 11	266, 205, 156, 31, 11
9	T	A15607G	AluI	15372F	16067R	TAGGAATCACCTCCCATTCC	GTCAATACTTGGGTGGTACC	696	236, 218, 170, 72	406, 218, 72
10	T	G13368A	BamHI	12951F	13614F	CGCTAATCCAAGCCTCACC	TATTCGAGTGCTATAGGCGC	691	691	416, 248
11	J	G13708A	BstNI	13568F	14276R	TTACTCTCATCGCTACCTCC	GGTTGATTCGGGAGGATCC	709	709	571, 138
12	J	C16069T	Hinf1	15879F	16545R	AATGGGCCTGTCCTTGTAG	AACGTGTGGGCTATTTAGGC	667	480, 122, 65	602, 65
13	V	G4580A	NlaIII	4184F	4869R	TCCTACCACTCACCCTAGC	GTCATGTGAGAAGAAGCA	686	684, 2	397, 287, 2
14	X	G1719A	DdeI	1138F	1801R	GAACACTACGAGCCACAGC	TCATCTTTCCCTTGCGGTAC	664	188, 187, 134, 111, 30, 14	188, 187, 111, 86, 48, 30, 14
15	W1	A11947G	BsmFI	11765F	12108R	GCACTCACAGTCGCATCATAA	TTGAGGGATAGGAGGAGAATG	343	196, 149	343
16	M	C10400T	AluI	10381F*	10671R	AAAAAGGATTAGACTGAGCTGA	CGGCAAAGACTAGTATGGCAA	318	201, 172, 18	219, 72
17	J, T, L2, H	T4216C	NlaIII	4142F	4379R	GATTCCGCTACGACCAACTC	GCACGGAGAATTTTGGATTC	197	160, 78	197
18	HV	C14766T	MseI	14642F	14968R	CCCACACTCAACAGAAACAAA	AGCGGATGATTCAGCCATAA	346	203, 142, 4	203, 125, 17, 4
19	U/K	A1811G	PsiI	1623F	1909R	GCACCCAACTTACACTTAGGA	TTTCGGGGGTCTTAGCTTT	287	287	188, 101
20	K	A10550G	NlaIII	10387F	10761R	GATTAGACTGAACCGAATTGG	CGGCAAAGACTAGTATGGCAA	285	164, 121	285
21	T	A4917G	BfaI	4865F	5192R	ATGACAAAAACTAGCCCCCA	AGGGTGGATGGAATTAAGGGT	348	298, 39, 11	337, 11
22	X	T6221C	Mnl I	5881F	6254R*	GCCATTTTACCTCACCCCCACTGATGTTCG	TATAGCAGATGCGAGCAGGAGTAGGAGATAGGGA	374	131, 115, 108, 20	115, 108, 106, 25, 20

Restriction enzymes and primers used indicated. * mismatched primers.

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ISSN: 1423-0127

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