Figure 2
Caspase inhibitor reduces high cell density-induced MLL bcr cleavage. SUNE1 cell seeded at cell number of 0.4 × 105 (lanes 1 and 4), 2 × 105 (lanes 2 and 5) and 4 × 105 (lanes 3 and 6) were allowed to grow for 4 days in the absence (lanes 1-3) or presence (lanes 4-6) of 50 μM caspase-3 inhibitor II (Z-DEVD-FMK). Extracted genomic DNA was processed for Southern hybridization as described in methods. Arrows labeled 8.3 kb and 1.5 kb show the positions of the intact and the cleaved MLL bcr respectively. MDig represents the DIG-labeled DNA marker.