Figure 2

GTH regulates cytokine mRNA expression. RAW264.7 cells were incubated with GTH at 20, 40, 80, and 165 μg/ml for 24 hrs and stimulated with GTH LPS (10 ng/ml) for 4 hrs. (A) RT-PCR for mRNA expression of RAW264.7 cells treated with different concentrations of GTH, (B) IL-1β, (C) IL-6, (D) iNOS and (E) TNF-α mRNA at GTH 165 μg/m was quantified. Values represent mean ± SEM (n = 3, * = P < 0.05 ** = P < 0.001 compared with LPS alone)