Table 1 Primers used and their sequences
From: A role of ygfZ in the Escherichia coli response to plumbagin challenge
Name | Sequence (5' to 3') | Used in construction |
|---|---|---|
PygfZF | CCATGGCTTTTACACCTTTTCCTCCCCG | pQE-ygfZ |
PygfZR | AGATCTCTCTTCGAGCGAATACGGCAGC | |
PsodAF | GGACTTATGAGCTATACCCTGCCATC | pQE-sodA |
PsodAR | GGATCCTTTTTTCGCCGCAAAACGTA | |
PkpygfZF | CCATGGGTATGGCTTTTACACCTTTTCC | pQE-Kp_ygfZ |
PkpygfZR | AGATCTATTTTCTTCCAGCGAATACGGC | |
PRv0811cF | CCATGGCCGCAGTCCCTGCCCCAGACCC | pQE-Rv_0811c |
PRv0811cR | AGATCTCCGAATACCGCCGCGCAGCCGC | |
PygfZK226AF | CAGCTTTAAGGCCGGCTGTTATACCG | pQE-ygfZK226A |
PygfZk226AR | CGGTATAACAGCCGGCCTTAAAGCTG | |
PygfZG227AF | CTTTAAGAAAGCCTGTTATACCGGAC | pQE-ygfZG227A |
PygfZG227AR | GTCCGGTATAACAGGCTTTCTTAAAG | |
PygfZC228AF | CTTTAAGAAAGGGGCTTATACCGGACAAG | pQE-ygfZC228A |
PygfZC228AR | CTTGTCCGGTATAAGCCCCTTTCTTAAAG | |
PygfZC228SF | CTTTAAGAAAGGCTCGTATACCGGAC | pQE-ygfZC228S |
PygfZC228SR | GTCCGGTATACGAGCCTTTCTTAAAG | |
PygfZC228MF | CTTTAAGAAAGGCATGTATACCGGAC | pQE-ygfZC228M |
PygfZC228MR | GTCCGGTATACATGCCTTTCTTAAAG | |
PygfZY229AF | TAAGAAAGGCTGTGCTACCGGACAAG | pQE-ygfZY229A |
PygfZY229AR | CTTGTCCGGTAGCACAGCCTTTCTTA | |
PygfZT230AF | AAGGCTGTTATGCCGGACAAGAGATG | pQE-ygfZT230A |
PygfZT230AR | CATCTCTTGTCCGGCATAACAGCCTT | |
PygfZG231AF | GCTGTTATACCGCGCAAGAGATGGTG | pQE-ygfZG231A |
PygfZG231AR | CACCATCTCTTGCGCGGTATAACAGC | |
PygfZQ232AF | CTGTTATACCGGAGCAGAGATGGTGG | pQE-ygfZQ232A |
PygfZQ232AR | CCACCATCTCTGCTCCGGTATAACAG | |
PygfZE233AF | GTTATACCGGACAGGCCATGGTGGCGCGA | pQE-ygfZE233A |
PygfZE233AR | TCGCGCCACCATGGCCTGTCCGGTATAAC | |
PygfZΔ226-237F | GGGCGGTATCAGCTTTAAGGCCAAATTCC | pQE-ygfZΔ226-237 |
PygfZΔ226-237R | GGAATTTGGCCTTAAAGCTGATACCGCCC | |
PQEF | GGCGTATCACGAGGCCCTTTTCG | Fragment amplification |
PQER | CATTACTGGATCTATCAACAGG | Fragment amplification |