Figure 1From: Prenatal exposure of ethanol induces increased glutamatergic neuronal differentiation of neural progenitor cellsEthanol inhibited the proliferation of NPCs. We treated two concentrations (10 mM and 50 mM) of ethanol to rat primary NPCs culture for 1 or 3 days. Cell viability (A) and BrdU incorporation (B) was examined as described in methods. (A) MTT analysis. Ethanol did not induce cellular toxicity against NPCs. (B) Both on day 1 and 3, BrdU incorporation was inhibited by ethanol treatment in a concentration-dependent manner. (C) To investigate inhibitory effect of ethanol on cell proliferation, immunocytochemistry against pH3 or PCNA was performed on day 3. The number of pH3-positive cells as well as PCNA positive cells was reduced by ethanol treatment. (D) FACS analysis of cell cycle. FACS analysis was performed as described in methods 4 hr after ethanol treatment on NPCs culture. Ethanol treatment decreased cells in G2/M phase as compared with control. Values are expressed as the mean ± S.E.M. **, *** p < 0.01 and < 0.001 vs. control (n = 5 for A, B and C. n = 3 for D).Back to article page