|
NIS mutants
|
V maxa
|
K ma
|
|---|
|
Wild type
|
7.94 ± 0.2
|
81.35 ± 8.89
|
|
R82A
|
2.46 ± 0.31***
|
32.87 ± 5.58**
|
|
K86A
|
5.49 ± 0.61**
|
68.97 ± 14.08
|
|
H226A
|
2.02 ± 0.48***
|
71.37 ± 12.43
|
|
R228A
|
4.67 ± 0.45***
|
91.8 ± 7.2
|
|
R239A
|
2.42 ± 0.45***
|
171.23 ± 36.12**
|
|
R241A
|
2.15 ± 0.14***
|
67.64 ± 14.69
|
|
E79A
|
8.55 ± 0.46
|
110.42 ± 39.87
|
|
D163A
|
9.46 ± 3.1
|
166.51 ± 37.9*
|
|
D233A
|
8.37 ± 1.6
|
207.49 ± 72.65*
|
|
D237A
|
7.93 ± 0.52
|
133.46 ± 43.93*
|
|
D311A
|
6.37 ± 0.69
|
59.89 ± 16.84
|
|
D322A
|
9.45 ± 1.11
|
496.6 ± 67.35***
|
|
D331A
|
3.98 ± 0.72***
|
91.27 ± 15.32
|
- a Values are mean ± standard error of triplicate assays.
- *p < 0.05, **p < 0.01, ***p < 0.001, compared with wild type.
