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Table 1 Kinetic analysis of human NIS mutants.

From: Conserved charged amino acid residues in the extracellular region of sodium/iodide symporter are critical for iodide transport activity

NIS mutants

V maxa

K ma

Wild type

7.94 ± 0.2

81.35 ± 8.89

R82A

2.46 ± 0.31***

32.87 ± 5.58**

K86A

5.49 ± 0.61**

68.97 ± 14.08

H226A

2.02 ± 0.48***

71.37 ± 12.43

R228A

4.67 ± 0.45***

91.8 ± 7.2

R239A

2.42 ± 0.45***

171.23 ± 36.12**

R241A

2.15 ± 0.14***

67.64 ± 14.69

E79A

8.55 ± 0.46

110.42 ± 39.87

D163A

9.46 ± 3.1

166.51 ± 37.9*

D233A

8.37 ± 1.6

207.49 ± 72.65*

D237A

7.93 ± 0.52

133.46 ± 43.93*

D311A

6.37 ± 0.69

59.89 ± 16.84

D322A

9.45 ± 1.11

496.6 ± 67.35***

D331A

3.98 ± 0.72***

91.27 ± 15.32

  1. a Values are mean ± standard error of triplicate assays.
  2. *p < 0.05, **p < 0.01, ***p < 0.001, compared with wild type.