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Table 1 Kinetic analysis of human NIS mutants.

From: Conserved charged amino acid residues in the extracellular region of sodium/iodide symporter are critical for iodide transport activity

NIS mutants V maxa K ma
Wild type 7.94 ± 0.2 81.35 ± 8.89
R82A 2.46 ± 0.31*** 32.87 ± 5.58**
K86A 5.49 ± 0.61** 68.97 ± 14.08
H226A 2.02 ± 0.48*** 71.37 ± 12.43
R228A 4.67 ± 0.45*** 91.8 ± 7.2
R239A 2.42 ± 0.45*** 171.23 ± 36.12**
R241A 2.15 ± 0.14*** 67.64 ± 14.69
E79A 8.55 ± 0.46 110.42 ± 39.87
D163A 9.46 ± 3.1 166.51 ± 37.9*
D233A 8.37 ± 1.6 207.49 ± 72.65*
D237A 7.93 ± 0.52 133.46 ± 43.93*
D311A 6.37 ± 0.69 59.89 ± 16.84
D322A 9.45 ± 1.11 496.6 ± 67.35***
D331A 3.98 ± 0.72*** 91.27 ± 15.32
  1. a Values are mean ± standard error of triplicate assays.
  2. *p < 0.05, **p < 0.01, ***p < 0.001, compared with wild type.