GLUT1 immunostaining in normal mice. Cortex immunostaining: cells within layers I to IV exhibit a cytoplasmic staining. The staining is presented as follows: A: Alexa 488 signals (green) for GLUT1. The arrow indicates an example of stained cell; B: Hoecsht signals (blue) for the nuclear counterstaining; C: Alexa 488 signals (green) and Hoechst signals (blue) are merged; D: Corpus callosum (CC) staining: a few stained oligodendrocytes are seen (arrow). (Alexa 488 signal and Hoechst signals merged); E: Striatum staining: GLUT1 staining appears homogeneous and weak with few cellular bodies stained. The white-matter tracts are not labeled for GLUT1. (Alexa 488 signal and Hoechst signals merged); F: Cerebellum staining: The granular layer (GL) and the molecular layer (ML) are irregularly labelled for GLUT1, whereas the molecular layer is homogeneously labelled for PiT1 and PiT2. (Alexa 488 signal and Hoechst signals merged). Scale bar: 100 μm.