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Table 2 Effect of oxidative stress inducing agents on [3H]taurine uptake in TR-TBT cells

From: Regulation of taurine transport at the blood-placental barrier by calcium ion, PKC activator and oxidative stress conditions

Pretreatment [3H]Taurine uptake (% of control)
Compounds TR-TBT 18d-1 TR-TBT 18d-2
Control 100 ± 7 100 ± 14
TNF- α 20 ng/mL 139 ± 11 * 150 ± 12 **
LPS 10 ng/mL 124 ± 4 ** 128 ± 7 *
DEM 100 μM 137 ± 16 ** 154 ± 11 **
H2O2 2 mM 85.2 ± 1.8 ** 79.8 ± 3.2 **
SIN-1 1 mM 66.8 ± 2.2 ** 84.2 ± 3.8 *
  1. Cells were treated with tumor necrosis factor-α (TNF- α, 20 ng/mL), lipopolysaccharide (LPS, 10 ng/mL), diethyl maleate (DEM, 100 μM), hydrogen peroxide (H2O2, 2 mM) and 3-morpholinosyndomine (SIN-1, 1 mM) for 24 hrs, respectively. [3H]Taurine (28 nM) uptake was performed at 37°C for 5 min. Each value represents the mean ± SEM (n=3-4). *p<0.05, ** p<0.01 significantly different from the control.
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