NBS1 overexpression induced in vitro migration and invasion activity of H1299 cells and the expression of HSPA4 and HSPA14. (A) Western blot analysis of NBS1 levels in H1299NBS1 clones vs. H1299 control clones. (B) NBS1 overexpression increased the migration and invasion activity of H1299 cells. The asterisk (*) indicated statistical significance (P < 0.05) between H1299NBS1 and H1299-control clones. (C) Induction of HSPA4 and HSPA14 expression by NBS1 overexpression in two different cell lines (FADU control vs. FADUNBS1, H1299 control vs. H1299NBS1).