Figure 3
From: Functional characterization of Trip10 in cancer cell growth and survival
Trip10 interacts with both Cdc42 and huntingtin (HD) and shows cell type-specific localization. Trip10 was cloned and transfected into (A) IMR-32 cells and (B) CP70 cells; individual colonies were selected and analyzed by Western blot (top panels). Interactions of Trip10 with Cdc42 and HD were analyzed by immunoprecipitation. After immunoprecipitation of Trip10, the protein complex was probed with Cdc42 and HD antibodies (middle panels). Immunostaining (bottom panels) show the distribution of Trip10 and HD. Vehicle: empty vector only; Ctrl: transfection agent only.