Reduced SRF protein levels in mouse hearts resulted in gene expression patterns that were opposite to what was observed in SRF-Tg heart. 5A. The up-regulation of miR-21, and the down-regulation of miR-1 and miR-133a were observed in SRF-Tg compared to wild-type (WT) mouse heart (P < 0.01**, n = 3). Interestingly, the down-regulation of miR-21, but up-regulation of miR-1 and mir-133a were observed in Anti-SRF-Tg compared to wild-type mouse heart (p < 0.01**, n = 3). 5B. Myocardin, Zipzap, p49/Strap and ANF were up-regulated in the SRF-Tg compared with the wild-type mouse heart, but down-regulated in the Anti-SRF-Tg compared with the wild-type mouse heart (p < 0.01**, n = 3).