Figure 1

The calcium sensing receptor (CaSR) is expressed in pulmonary artery smooth muscle cells (PASMCs) and homogenates of pulmonary arteries (PAs). A. Detection of CaSR mRNA by RT-PCR in rat PASMCs in the absence or presence of reverse transcriptase and GAPDH. B. Detection of CaSR protein by western blotting in rat cultured PASMCs and PAs. Positive and negative control from rat cardiac tissue (left) and the specific antigenic peptides (right) are also shown. C. Immunofluorescence detection of CaSR in rat PASMCs in the presence of anti-CaSR Ig conjugated with FITC (left) and in the presence of specific antigenic peptides and anti-CaSR Ig (right), (magnification: 400 ×). D. Immunofluorescence detection of CaSR in rat PAs in the presence of anti-CaSR Ig conjugated with FITC (left) and in the presence of specific antigenic peptides and anti-CaSR Ig (right) (magnification: 200 ×), bar = 50 μM.