Figure 1

Etoposide (ETO)-induced cell death and FMRP induction in HeLa cells. (A) HeLa cells were treated with 1-200 μM ETO for 3 hr, then cell viability was analyzed by MTT assay. (B) Fmr1 mRNA level was analyzed by reverse transcription polymerase chain reaction (RT-PCR) procedures. For comparison, PCR reaction for housekeeping gene, GAPDH, was also performed. (C) The increase of FMRP protein was analyzed by Western blot and β-actin was used as a loading control. (D) ETO-induced expression of FMRP was visualized by immunocytochemistry. Blue fluorescence represents DAPI staining and green fluorescence means FMRP. Each graph represents quantification of RT-PCR and Western blot band intensity, respectively. Data represent mean ± S.E.M. * significantly different as compared with control and ^# significantly different as compared with ETO alone treatment (p < 0.01, n = 4).