Expression of Fas (A), Fas-L (B) and caspase-3 (C) determined by Western blot analysis. The intensity of each band was quantified by densitometry, and data were normalized to the β-actin signal. The expression levels in the control group were considered the basal levels, and the others are expressed as fold change from the control group. The fold change values represent the means ± S.E.M. of five determinations. ▼▼P < 0.01 versus control; ★P < 0.05, ★★P < 0.01 versus ischemia/reperfusion.