Apoptosis and iron deposition/infiltration of cardiomyocytes following simvastatin treatment in I+G mice. (A and C) Apoptotic cardiac myocytes were detected by the TUNEL assay in control group, I+G group, and I+G with simvastatin (I+G+St) treatment group respectively. Left and right panels show the TUNEL positive (green) and nuclei (blue) fluorescence, respectively. Each histogram represents the number of TUNEL-positive cells in each group (n = 5 animals in each group). (B and D) Iron deposition/infiltration in cardiac tissue for each group was stained and quantified. Representative results of three separate experiments are shown. Bar = 200 μm; **p < 0.001 vs control; ††p < 0.001 vs I+G.