Aciculatin suppresses NF-κB activation in LPS-activated macrophages. A. RAW264.7 macrophages (1 × 106 cells) were treated with aciculatin (3, 10 μM) for 30 min and stimulated with LPS (1 μg/mL) for 24 h. Then the cells were harvested and whole cell extracts were prepared for Western blot analysis for the indicated proteins. B. Cells (1 × 105 cells) were transiently transfected with 1 μg of pGL4.32[luc2P/ NF-κB-RE/Hygro] for 24 h and treated with 1-10 μM aciculatin for 30 min before stimulation with LPS (1 μg/mL) for a further 24 h. Luciferase activity was measured as described in the Materials and Methods. The results are expressed as the mean ± S.E.M. for three separate experiments, each with three replicates. ** p < 0.01 compared to the control group; # p < 0.05 and ## p < 0.01 for comparison of indicated groups.